Evaluation of Inflammatory Markers in Samples

The following assays were conducted: the General Nicotinamide Adenine Dinucleotide (NAD) ELISA Kit (Ref: MBS2700640-96, MyBioSource, San Diego, California, UnitedStates), to detect the amount of NAD; the Citrullinated Histone H3 (Clone 11D3) ELISA Kit (Ref: 501620, Lot: 0637729, Cayman Chemical Company, Ann Arbor, Michigan, United States), to detect the amount of citrullinated histone H3 (H3Cit); the Cell Death Detection ELISA^PLUS Kit (Ref: 11774425001, Sigma), to detect the amount of nucleosomes; the Porcine IFN gamma (γ) ELISA Kit (Ref: KSC4021, Thermo Fisher Scientific Inc., Waltham, Massachusetts, United States), to detect IFN-γ; and the Porcine IFN alpha (α) ELISA Kit (Ref: ES7RB, Thermo Fisher Scientific Inc.), to detect IFN-α. The assays were performed according to the manufacturer’s recommendations. A sandwich ELISA for the detection of histone–MPO complexes was performed as described before (26 (link)).