The study was conducted at seven hospitals in Federal Capital Territory (FCT) and three in Kano Nigeria as previously described from Sept 2008—Dec 2016 [1 (link), 23 (link)]. Children aged less than five years were enrolled at the different sites, blood specimens of 1–3 ml were collected using a vacutainer set, after aseptically cleansing the skin with alcohol swab and povidone-iodine, the specimen was collected directly into an aerobic blood culture bottle (BD BactecPeds Plus/F culture vials; Becton Dickinson, Ireland), and incubated in an automated Bactec® 9050 machine. All positive bottles were sub cultured onto MacConkey, Sheep blood and chocolate agar plates at 36°C for 24 h.
A total number of 887 culture-positive Enterobacteriaceae were obtained. Of the 887 isolates, 474 salmonella species including Typhi which have been reported by Obaro et al. 2015 were excluded [23 (link)], therefore 413 including Escherichia coli, Klebsiella species, Enterobacter species, Serratia marcescens, Pantoea species, Salmonella Typhi and Citrobacter species from September 2008 to December 2016 were included. The isolates were stored in 10% skim milk glycerol at -80°C.
A total number of 887 culture-positive Enterobacteriaceae were obtained. Of the 887 isolates, 474 salmonella species including Typhi which have been reported by Obaro et al. 2015 were excluded [23 (link)], therefore 413 including Escherichia coli, Klebsiella species, Enterobacter species, Serratia marcescens, Pantoea species, Salmonella Typhi and Citrobacter species from September 2008 to December 2016 were included. The isolates were stored in 10% skim milk glycerol at -80°C.
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