Western Blot for ACE2, TMPRSS2, and GAPDH

Antibodies against ACE2 (ab15348), TMPRSS2 (ab109131), and GAPDH (ab8245) were obtained from Abcam (Cambridge, UK). Western blot samples were prepared as described previously [66 (link)]. Proteins on nitrocellulose membranes were visualized with the biotin/streptavidin-horseradish peroxidase system (GE Healthcare; Little Chalfont, UK) in combination with the “Renaissance Western Blot Chemoluminescence Reagent” (Perkin Elmer; Waltham, MA, USA). Documentation was performed using the digital system ChemoStar Imager (INTAS, Göttingen, Germany).

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Pommerenke C., Rand U., Uphoff C.C., Nagel S., Zaborski M., Hauer V., Kaufmann M., Meyer C., Denkmann S.A., Riese P., Eschke K., Kim Y., Safranko Z.M., Kurolt I.C., Markotic A., Cicin-Sain L, & Steenpass L. (2021). Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies. PLoS ONE, 16(8), e0255622.

Publication 2021

ab15348 ab109131 ab8245 biotin/streptavidin-horseradish peroxidase system Renaissance Western Blot Chemoluminescence Reagent ChemoStar Imager

Ace2 Antibodies Biotin Gapdh Horseradish peroxidase Imager Membranes proteins Nitrocellulose Streptavidin Tmprss2 Western blot

Corresponding Organization : Centre for Individualised Infection Medicine

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Variable analysis

independent variables

Antibodies against ACE2 (ab15348), TMPRSS2 (ab109131), and GAPDH (ab8245) obtained from Abcam (Cambridge, UK)

dependent variables

Protein expression levels on nitrocellulose membranes

control variables

Western blot samples prepared as described previously [66]

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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