All samples were amplified for the 23 Y‐STRs loci included in the PowerPlex® Y23 System (Promega) following manufacturer recommended protocols. PCR products were sized on an ABI PRISM 310 Genetic Analyzer and alleles were called with GeneMapper ID software (Thermo Fisher Scientific) according to the manufacturer's instructions.
Next, all individuals were additionally genotyped for 42 Y‐SNP loci using multiplex SNaPshot mini‐sequencing assays (Thermo Fisher Scientific), as described in Sarno et al. (2014 (link)). The SNP genotyping was carried out by means of PCR Multiplex amplification, followed by Minisequencing reaction based on dideoxy Single Base Extension (SBE), which was performed with the SNaPshot multiplex kit (Applied Biosystems). SBE products were finally analyzed through capillary electrophoresis on an ABI Prism 310 Genetic Analyser.
Y‐STRs data for comparison populations were extracted from the literature for both Italy (Boattini et al., 2013 (link)) and Europe (Purps et al., 2014 (link)), respectively. In addition, comparison data for within‐haplogroup comparisons from 16 Euro‐Mediterranean populations (329 samples) were also considered and retrieved from Hallast et al. (2015 (link)).
Next, all individuals were additionally genotyped for 42 Y‐SNP loci using multiplex SNaPshot mini‐sequencing assays (Thermo Fisher Scientific), as described in Sarno et al. (2014 (link)). The SNP genotyping was carried out by means of PCR Multiplex amplification, followed by Minisequencing reaction based on dideoxy Single Base Extension (SBE), which was performed with the SNaPshot multiplex kit (Applied Biosystems). SBE products were finally analyzed through capillary electrophoresis on an ABI Prism 310 Genetic Analyser.
Y‐STRs data for comparison populations were extracted from the literature for both Italy (Boattini et al., 2013 (link)) and Europe (Purps et al., 2014 (link)), respectively. In addition, comparison data for within‐haplogroup comparisons from 16 Euro‐Mediterranean populations (329 samples) were also considered and retrieved from Hallast et al. (2015 (link)).
Free full text: Click here
