Single-cell topographical and mechanical characteristics of living CAL27 cells were measured by a AFM nano-indentation method (JPK Instruments AG, Germany), as described previously (26 (link), 27 (link)). Adhesion energy in the cytoplasmic regions was obtained using the JPK data processing software (Version spm-4.2.50, JPK, Germany) (28 (link), 29 (link)).
Free full text: Click here