Fibroblast Cell Activation and Inhibition

HDF was bought from American Type Culture Collection (Cat# PCS-201-012, cell type: fibroblast, origin: human skin) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fatal bovine serum at 37°C, 5% CO₂ atmosphere (Gusarov et al., 2021 (link)). Lipopolysaccharide (Cat# L4391, e. coli. 0111: B4, Merck, Germany) was used to stimulate HDF in vitro. Taxol (1 μM, Cat# S1150, Selleck, United States) or SB203580 (5 μM, Cat# S1076, Selleck, United States) was used to the culture and pretreated at 37°C for 1 h before LPS stimulation.

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Cen R., Wang L., He Y., Yue C., Tan Y., Li L, & Lei X. (2022). Dermal Fibroblast Migration and Proliferation Upon Wounding or Lipopolysaccharide Exposure is Mediated by Stathmin. Frontiers in Pharmacology, 12, 781282.

Publication 2021

Lipopolysaccharide Taxol SB203580

Atmosphere Bovine Cell type Cultured medium E coli Eagle Fibroblast Human Sb203580 Serum Skin Taxol

Corresponding Organization :

Other organizations : Daping Hospital, Army Medical University

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Variable analysis

independent variables

Lipopolysaccharide (LPS, Cat# L4391, e. coli 0111: B4, Merck, Germany)
Taxol (1 μM, Cat# S1150, Selleck, United States)
SB203580 (5 μM, Cat# S1076, Selleck, United States)

dependent variables

Not explicitly mentioned

control variables

Cell type: fibroblast
Origin: human skin
Culture medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fatal bovine serum
Temperature: 37°C
Atmosphere: 5% CO2

controls

Positive control: Not specified
Negative control: Not specified

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