Dental Plaque DNA Extraction Protocol
Corresponding Organization : Meharry Medical College
Variable analysis
- None explicitly mentioned
- Bacterial DNA collected from dental plaque samples
- Dental plaque samples collected by board-certified periodontists
- Sterile paper points used for sample collection
- Samples collected at baseline prior to any dental treatment
- Samples collected from the mesiobuccal sulci of the first molar in each quadrant
- Samples collected for 1 minute
- Samples immediately immersed in Tris-EDTA (TE) buffer (pH 7.5)
- Bacterial cells harvested by centrifugation
- Bacterial pellet resuspended in 100 µl TE buffer
- Bacterial chromosomal DNA released by freezing and boiling
- No positive or negative controls were explicitly mentioned in the provided information
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!