Cultivation of Cancer Cell Lines

Ovarian cancer cell lines A2780 and SKOV-3 were obtained from the institutional Cell Line Core Laboratory of the MD Anderson Cancer Center. Prostate cancer cell lines LNCaP and PC-3 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), as well as the NIH3T3 normal cell line. Ovarian cell lines were grown in RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 15% fetal bovine serum (FBS; Life Technologies, Carlsbad, CA, USA), 2.0 g sodium bicarbonate, 0.1% gentamicin solution. LNCaP cells were cultured in RPMI-1640 supplemented with 10% FBS, 1.5 g sodium bicarbonate, 2.5 g glucose, 2.383 g HEPES, 0.11 g sodium pyruvate and 1% penicillin/streptomycin. The PC-3 cell line was grown in DMEM (high glucose) complemented with 10% FBS, 1.5 g sodium bicarbonate, 0.22 g sodium pyruvate and 1% penicillin/streptomycin. NIH3T3 cell line was cultured in DMEM (high glucose) supplemented with 10% FBS, 2.7 g sodium bicarbonate and 1% penicillin/streptomycin. All cell lines were incubated at 37 °C in 5% CO₂. All the other reagents were purchased from Sigma-Aldrich. The cell lines were certified free of mycoplasma (read B/read A ratio below 0.9; see figure S7 in Supplementary Information) using the MycoAlert^® mycoplasma detection kit and MycoAlert^® assay control set (Lonza, Morristown, NJ, USA). Subsection C in Supplementary Information provides details regarding the management and cell counting of these cell lines.