Tumor Dissociation and Cell Analysis

Tumors were excised and digested postmortem using a cocktail of 1 mg/ml collagenase type IV (Sigma-Aldrich) and 0.02 mg/mL DNaseI (Sigma-Aldrich). After digestion at 37 °C for 30 min, cells were passed through a 70 μm filter twice. Cells were then analyzed for various functional parameters including cytokine production by flow cytometry directly ex vivo as previously described [41 (link)].

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Guo D., Jin C., Gao Y., Lin H., Zhang L., Zhou Y., Yao J., Duan Y., Ren Y., Hui X., Ge Y., Yang R, & Jiang W. (2023). GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint. Cell & Bioscience, 13, 51.

Publication 2023

collagenase type IV DNaseI

Cells Cytokine Digestion Flow cytometry Postmortem Tumors Type iv collagenase

Corresponding Organization :

Other organizations : East China Normal University

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Variable analysis

independent variables

Tumor digestion using a cocktail of 1 mg/ml collagenase type IV (Sigma-Aldrich) and 0.02 mg/mL DNaseI (Sigma-Aldrich)

dependent variables

Various functional parameters including cytokine production

control variables

Digestion time (30 min)
Temperature (37 °C)
Cell filtration (70 μm filter)

controls

No positive or negative controls were explicitly mentioned.

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