Urine was collected during the 24-h period according to the given instructions outlined by the WHO [37 ] in a standard, sterilized urine collection bottle. Measured parameters included 24-h urine sodium, potassium, creatinine coefficients and protein concentrations. Sodium and potassium molar excretion in 24-h urine was used for the estimation of daily sodium and potassium consumption in milligrams (mg) using appropriate formulas [1 mmol = 22.99 mg of sodium or 39.10 mg of potassium], and daily salt intake was estimated based on 24-h urinary sodium excretion [1 g salt (NaCl) = 393.4 mg Na = 17.1 mmol Na]; salt intake can then be calculated: salt (g/day) = 24-h urinary sodium (mmol/24-h)/17.1) [38 (link)]. The sodium to potassium ratio was also calculated. The 24-h urine samples were analyzed at the Clinical Institute of Laboratory Diagnostics, University Hospital Osijek, Osijek, Croatia.
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