BG505 SOSIP MD39 (250 μg) was incubated with 600 μg of Rh.33104 mAb.1 Fab and 600 μg of RM20A3 Fab (6 (link), 46 (link)) at room temperature overnight. The complex was SEC purified using a HiLoad 16/600 Superdex pg200 (GE Healthcare) column, with TBS as a running buffer. SEC fractions corresponding to the complex were pooled and concentrated to 6.0 mg/ml using an Amicon filter unit with 10-kDa cutoff (EMD Millipore).
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