siRNA Transfection for FANC Knockdown

All siRNA duplexes used in this study were purchased from Invitrogen or Sigma. Transfection and co-transfection was carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Cells were harvested 72 h after transfection. The individual siRNA duplexes used were: siFANCD2 (5′CAGAGUUUGCUUCACUCUCUATT-3′) (Invitrogen) (4 (link)) siFANCA (5′AAGGGUCAAGAGGGAAAAAUA-3′) (Invitrogen) (5 (link)); Luciferase Control (Ctrl) (5′UCGAAGUAUUCCGCGUACGTT-3′) (Invitrogen) (8 (link)).

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Okamoto Y., Iwasaki W.M., Kugou K., Takahashi K.K., Oda A., Sato K., Kobayashi W., Kawai H., Sakasai R., Takaori-Kondo A., Yamamoto T., Kanemaki M.T., Taoka M., Isobe T., Kurumizaka H., Innan H., Ohta K., Ishiai M, & Takata M. (2018). Replication stress induces accumulation of FANCD2 at central region of large fragile genes. Nucleic Acids Research, 46(6), 2932-2944.

Publication 2018

Lipofectamine RNAiMAX siFANCD2

Cells Lipofectamine Luciferase Sirna Transfection

Corresponding Organization : Kyoto University

Other organizations : The Graduate University for Advanced Studies, SOKENDAI, Tokyo University of the Arts, The University of Tokyo, Waseda University, Hiroshima University, Kanazawa Medical University, National Institute of Genetics, Research Organization of Information and Systems, Tokyo Metropolitan University

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Variable analysis

independent variables

SiRNA duplexes used in the study, including siFANCD2, siFANCA, and Luciferase Control (Ctrl)

dependent variables

Unspecified, as the outcome measurements are not explicitly mentioned

control variables

Transfection and co-transfection carried out using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions
Cells harvested 72 h after transfection

controls

Positive control: Luciferase Control (Ctrl) siRNA
Negative control: Not explicitly mentioned

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