Human HEK293T cells purchased from the ATCC, and stat3-null HeLa cells purchased from ABclonal (20170728-02) were grown in Dulbecco's modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS; FND500, excellbio), 100 U/ml penicillin and 100 U/ml streptomycin. GSC lines were obtained from Professor Jeremy N. Rich (University of California San Diego). GSCs were cultured in Neurobasal™ medium (12348017, Gibco) supplied with B27 supplement (12587010, Gibco), 10 ng/ml epidermal growth factor (EGF) and 10 ng/ml fibroblast growth factor (FGF) as previously described (17 (link),18 (link)). All cell lines were used for a limited number of passages and checked for Mycoplasma infection.
Protocol detail
Cell Culture Protocols for HEK293T, HeLa, and GSC Lines
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Cell lines
Cells
Cultured medium
Eagle
Epidermal growth factor
Hela
Human
Mycoplasma infection
Null cells
Penicillin
Stat3
Streptomycin
Supplement
Corresponding Organization : National Center of Biomedical Analysis
Other organizations : Capital Medical University, Beijing Children’s Hospital
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Variable analysis
independent variables
- Cell type (Human HEK293T cells, stat3-null HeLa cells, GSC lines)
- Not explicitly mentioned
- Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 U/ml streptomycin
- Neurobasal™ medium supplied with B27 supplement, 10 ng/ml epidermal growth factor (EGF) and 10 ng/ml fibroblast growth factor (FGF)
- Positive control: Not specified
- Negative control: Not specified
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