All materials used were the same as previously reported.6 (link) Data for two IgG antibodies are reported: The monoclonal anti-FLAG M2 antibody produced in mouse was purchased from Sigma Aldrich and the monoclonal anti-M13 P8 antibody produced in mouse was purchased from Creative Diagnostics. The concentrations of the as-received antibody solutions were determined by performing Bradford Assays.
Protocol detail
Antibody Characterization Protocol
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Antibodies igg
Antibody
Assays
Diagnostics
Monoclonal antibody
Mouse
Corresponding Organization : University of California, Irvine
Other organizations : Sungkyunkwan University, Hangzhou Dianzi University
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Variable analysis
independent variables
- Monoclonal anti-FLAG M2 antibody produced in mouse
- Monoclonal anti-M13 P8 antibody produced in mouse
- Concentrations of the as-received antibody solutions
- All materials used were the same as previously reported.
- No positive or negative controls were explicitly mentioned.
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