Researchers performing quantification could not generally be blinded to experimental condition due to the overt changes in neuron numbers and brain structures induced by our manipulations. However, analysis was performed blind to the goals of the experiment when possible, and quantitation of features on the anterior and posterior sides of the brain were recorded independent of one another and merged after all quantifications were completed. Moreover, many of our analyses make use of variation within an experimental condition or genotype, providing an additional bulwark against observational bias.
Mushroom Body Anatomy Assessment
Researchers performing quantification could not generally be blinded to experimental condition due to the overt changes in neuron numbers and brain structures induced by our manipulations. However, analysis was performed blind to the goals of the experiment when possible, and quantitation of features on the anterior and posterior sides of the brain were recorded independent of one another and merged after all quantifications were completed. Moreover, many of our analyses make use of variation within an experimental condition or genotype, providing an additional bulwark against observational bias.
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Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : Janelia Research Campus, Howard Hughes Medical Institute, Johns Hopkins Medicine, Johns Hopkins University
Variable analysis
- Manipulations performed by researchers
- Immunohistochemistry
- Calyx area quantification
- Brp staining
- Sex differences in the fly, which are well-documented and have not been observed in the mushroom body
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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