Near-Infrared Imaging of Cartilage Tissue
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Corresponding Organization : Pusan National University
Other organizations : Chonnam National University, Curadel (United States)
Protocol cited in 2 other protocols
Variable analysis
- Time of paraformaldehyde fixation (30 min)
- Cartilage tissue morphology observed by NIR fluorescence microscopy
- Cartilage tissue staining with Alcian Blue
- Cartilage tissue staining with hematoxylin and eosin (H&E)
- Concentration of paraformaldehyde (2%)
- Mounting medium (Tissue-Tek OCT compound)
- Freezing method (flash-freezing in liquid nitrogen)
- Cryosectioning thickness (10 μm per slice)
- Microscopy system (4-filter Nikon Eclipse TE300 with 100 W mercury light source, NIR-compatible optics, and objective lenses)
- Image acquisition and analysis software (iVision)
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