RNA expression levels for GSK3β and APBB2 were obtained for the ROS/MAP from frozen sections of the dorsolateral prefrontal cortex that was manually dissected from postmortem brain tissue. Details of RNA extraction, processing and data quality control and normalization have been previously published (Lim et al. 2014 (link)). In brief, RNA was isolated using the RNeasy lipid tissue kit (Qiagen, Valencia, CA) and was reverse transcribed and biotin-UTP labeled using the llumina® TotalPrep™ RNA Amplification Kit from Ambion (Illumina, San Diego, CA). Expression signals were generated using the Beadstudio software suite (Illumina, San Diego, CA). Standard control and normalization methods were employed to account for technical variability due to differences in hybridization dates and stabilize the variance for the purpose of statistical analyses.
Protocol detail
Quantifying RNA Expression in Brain Tissue
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Biotin
Brain
Dorsolateral prefrontal cortex
Frozen sections
Gsk3β
Hybridization
Lipid
Postmortem
Rna expression
Tissue
Corresponding Organization :
Other organizations : Vanderbilt University Medical Center, Brigham and Women's Hospital, Case Western Reserve University, Harvard University, Rush University Medical Center
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Variable analysis
independent variables
- None explicitly mentioned
- RNA expression levels for GSK3β
- RNA expression levels for APBB2
- Differences in hybridization dates
- Technical variability
- Standard control and normalization methods were employed to account for technical variability and stabilize the variance for the purpose of statistical analyses.
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