Poloxamer 407 was purchased from BASF (Shanghai, China). Triethylamine, 4-nitrophenyl chloroformate, 4-dimethylaminopyridine, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were purchased from Aldrich Chemical Company (Shanghai, China). Low molecular weight heparin sodium salt (from porcine intestinal mucosa, 140 unit/mg) was supplied by Freda Biochem Company (Jinan, China). 2-octylcyanoacrylate was purchased from Beijing ShumKong Medical Company (Beijing, China). All other chemicals were used as received without further purification.
Japanese white rabbits (2-2.5 kg) were housed in an approved animal care center and were provided with standard chow and water ad libitum. The laboratory animal ethics committee and laboratory animal centre of Wenzhou Medical University approved all rabbit studies.
Free full text: Click here