Embryo Histology and Immunostaining

Embryos were collected and processed for histology, immunostaining, or in situ hybridization as described previously (Xu et al., 2016 (link)). For histology and immunofluorescent staining, the embryos were fixed in 4% paraformaldehyde (PFA), dehydrated through an ethanol series, embedded in paraffin, and sectioned at 7 μm thickness. The goat anti-Foxf1 (AF4798; R&D) antibody was used to detect the Foxf1 protein. Images were taken using a Nikon DS-Qi2 microscope (Nikon Instruments Inc., Melville, NY, United States).

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Xu J., Liu H., Lan Y, & Jiang R. (2021). Cis-Repression of Foxq1 Expression Affects Foxf2-Mediated Gene Expression in Palate Development. Frontiers in Cell and Developmental Biology, 9, 665109.

Publication 2021

AF4798 Nikon DS-Qi2 microscope

Antibody Dehydrated ethanol Embedded paraffin Embryos Goat Immunofluorescent In situ hybridization Microscope Paraformaldehyde Protein

Corresponding Organization : University of Cincinnati Medical Center

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Variable analysis

independent variables

Histology
Immunostaining
In situ hybridization

dependent variables

Expression of Foxf1 protein

control variables

Embryo fixation in 4% paraformaldehyde (PFA)
Dehydration through an ethanol series
Paraffin embedding
Sectioning at 7 μm thickness
Goat anti-Foxf1 (AF4798; R&D) antibody for Foxf1 protein detection
Imaging using a Nikon DS-Qi2 microscope

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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