Protocol detail

Grip Strength Evaluation of Neuroactive Compounds

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The effects of single doses of TC-G 1008, ZnCl₂, or VPA or repeated doses of these compounds and PTZ kindling on skeletal and muscular strength were evaluated in Swiss Albino or C57BL/6/Tar × CBA/Tar mice using the grip-strength test [46 (link)]. The grip-strength apparatus (BioSeb, Chaville, France) consisted of a steel wire grid (8 × 8 cm) connected to an isometric force transducer. A mouse was lifted by its tail to grasp the grid with its forepaws. The mouse was then gently pulled back until it released the grid, and the maximal force in newtons (N) exerted by the mouse before losing grip was measured. The procedure was repeated three times, and the mean force exerted by each mouse before losing grip was recorded. The mean force was normalized to body weight and expressed in mN/g ± SEM.

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Doboszewska U., Socała K., Pieróg M., Nieoczym D., Sawicki J., Szafarz M., Gawel K., Rafało-Ulińska A., Sajnóg A., Wyska E., Esguerra C.V., Szewczyk B., Maćkowiak M., Barałkiewicz D., Mlyniec K., Nowak G., Sowa I, & Wlaź P. (2023). TC-G 1008 facilitates epileptogenesis by acting selectively at the GPR39 receptor but non-selectively activates CREB in the hippocampus of pentylenetetrazole-kindled mice. Cellular and Molecular Life Sciences, 80(5), 133.

Publication 2023

grip-strength apparatus

Albino Body weight C57bl mice Grip Mouse Muscular strength Skeletal Steel Tail Tc g 1008 Transducer

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Variable analysis

independent variables

Single doses of TC-G 1008
Single doses of ZnCl2
Single doses of VPA
Repeated doses of TC-G 1008
Repeated doses of ZnCl2
Repeated doses of VPA
PTZ kindling

dependent variables

Skeletal and muscular strength, measured using the grip-strength test

control variables

Swiss Albino or C57BL/6/Tar × CBA/Tar mice
Grip-strength apparatus (BioSeb, Chaville, France) consisting of a steel wire grid (8 × 8 cm) connected to an isometric force transducer
Procedure of lifting the mouse by its tail to grasp the grid with its forepaws, then gently pulling back until it released the grid, and measuring the maximal force in newtons (N) exerted by the mouse before losing grip
Repeating the procedure three times and recording the mean force exerted by each mouse before losing grip
Normalizing the mean force to body weight and expressing it in mN/g ± SEM

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