Isolation and Identification of A. baumannii Strains

A total of 47 A. baumannii clinical isolates were derived from the blood, sputum or swab samples of patients from different departments at the First Affiliated Hospital of Chengdu Medical College, China from 2014 to 2015. Bacteria were aerobically grown at 37°C in Mueller-Hinton (MH) broth or agar (Oxoid, England) or in Luria-Bertani (LB) broth (Lin et al., 2009 (link)). These isolates were identified by ATB New (bioMérieux, France) (Chang et al., 2015 (link)) and further verified by PCR products of rpoB (Wang et al., 2014 (link)) and 16S rRNA gene (Chiang et al., 2011 (link)) with the primers included in Table S1. The PCR products were sequenced by Tsingke Biological Technology (Tsingke, Chengdu, China), followed by sequence alignment using BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi).