Co-immunoprecipitation (Co-IP) was performed as previously described [9 (link), 12 (link), 14 (link)], with antibodies specific for hnRNPU (ab10297), CTCF (ab188408), FLAG (ab125243), or Myc (ab9106, Abcam Inc.). Bead-bound proteins were released and analyzed by Western blot.
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