Plasma samples were obtained from 30-week-old control rats and from 27–32 week-old DM-cont and DM-empa rats after the 12-week treatment period. The plasma samples were treated with Phenomenex EZ:faast AA analysis kit (Phenomenex, Torrance, CA), with norvalin used as the internal standards [18 (link)]. GCMS-QP2010Plus and GCMS solution software (Shimadzu, Kyoto, Japan) were used for GC-MS analysis [19 (link)].
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