Anti-E-cadherin (HECD-1) antibody was from Invitrogen. Anti-CAR (H300), anti-TNFR1 (H5), anti-ICAM1 and anti-PKCδ antibodies were from Santa Cruz (Germany). Anti-phospho-PKCδ was from Cell Signalling Technology (USA). p-CAR thr290/ser293 polyclonal antibody was previously described in Morton et al.15 (link), and was developed by Perbioscience (Thermofisher) using the peptide Ac- RTS(pT)AR(pS)YIGSNH-C and was affinity purified before use. Anti-TNFR1 blocking antibody (Mab225) was obtained from R&D Systems (USA). Anti-GFP antibody was from Roche (UK). Anti-mouse HRP and anti-rabbit-HRP were from DAKO, anti-mouse-568, anti-rabbit-568 and phalloidin-633 were all obtained from Invitrogen (UK). CalyculinA, sodium orthovanadate and PI-3-kinase inhibitor (LY294002) were obtained from Calbiochem. TNFα, IL-5, IL-1β, IL-13 and IL-17 were obtained from Sigma-Aldrich (UK). FK was produced and purified as previously described35 (link). PKCδ targeted siRNA and non-targeting controls were obtained from Ambion (USA).
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