Measuring Epithelial Ion Transport

Electrophysiological experiments to measure the transport of ions across epithelial monolayers were accomplished using established methods [32 (link)]. Reagents were introduced into bath solution in the Ussing chamber (Physiologic Instruments, San Diego, CA, USA) in the following order: Amiloride, 100 μM (apical) to inhibit the ENaC current; forskolin, 10 µM (apical and basal) to stimulate CFTR; VX-770 10 µM (apical and basal) to further potentiate the current; and CFTRinh-172 10 µM (apical). Electrophysiological data were collected and analyzed using Acquire and Analyze 2.3 software (Physiologic Instruments, San Diego, CA, USA) [32 (link)].