Isolation and Sequencing of SIVmfa Genome

In 1987, PBMC from animal Mf186-76 were co-culture with H9 cells. A vial containing cell supernatant from this co-culture was obtained (a gift from R.C. Desrosiers). Viral RNA was isolated using the High Pure Viral RNA Kit (Roche USA, Indianapolis, IN). Amplicon-specific cDNAs were produced using the SuperScript^™ III One-Step RT-PCR System with Platinum^® Taq High Fidelity kit (Invitrogen, Carlsbad CA) using primers listed in S1 Table; amplicons were either sequenced directly, or TA-cloned using the TOPO TA cloning kit (Invitrogen, Carlsbad, CA) and then sequenced. The sequence of the full-length SIVmfa genome was deposited in GenBank under the accession number KU500642. The SIVmfa vif sequence was previously reported under KF030930 [22 (link)].