Wound Healing Assay for Trophoblast Cells
Corresponding Organization :
Other organizations : Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, International Peace Maternity & Child Health Hospital
Variable analysis
- Control siRNA
- SiUCA1 oligos
- Empty vector
- UCA1 overexpression vector
- Cell migration evaluated by comparison of the width of wound closure relative to the initial wound area at 0 h and 16 h
- 5 × 10^4 HTR-8 cells were plated into individual well of a 6-well plate and cultured for 24 h
- Cells were incubated with Mitomycin C (10 µg/mL) in DMEM/F12 medium for 2 h to eliminate cell proliferative ability
- Cells were cultured in DMEM/F12 medium with 1% FBS for another 16 h
- Positive control: Not specified
- Negative control: Control siRNA
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