Mouse spleen and eye infiltrating cell cultures were prepared and their intracellular expression of IL-17, IFN-γ, Τ−bet, or RORγt were determined as detailed elsewhere [23 , 26 (link)]. Briefly, the cells were incubated with ionomycin/PMA and Brefeldin A (GolgiPlug, BD Biosciences), followed by staining with conjugated anti-CD3 monoclonal antibody (145–2C11, BD Biosciences), anti-CD4 monoclonal antibody (RM4–5, Biolegend) and anti-CD45 monoclonal antibody (30-F11, Biolegend). After 4% paraformaldehyde fixation and permeabilization (BD bioscience) cells were intracellularly stained for T-bet (4B10, Biolegend), RORγt (AFKJS-9, BD Bioscience), IFN-γ (XMG1.2, Biolegend) and IL-17A (TC11–18H10.1, ebioscience). The staining patterns were analyzed by a MACSQuant Analyzer 10 (Miltenyi Bioitec. Cambridge, MA) and processed by FlowJo (Tree Star, Ashland, OR). Dead cells were excluded using ghost red 780 viability dye (Tonbo biosciences, San Diego, CA) and only single cells were gated for further analysis using FSC-A and FSC-H. Gating strategies are shown in supplementary Fig. S3 and Fig. S4.