Temporary (3 hour) middle cerebral artery occlusion (MCAO) was achieved using the intraluminal suture model6 (link) under isoflurane anesthesia, as we have previously reported 3 (link),4 (link). All animals were singly housed before and after surgery, with free access to food and water. At reperfusion, a single dose of candesartan 1mg/kg or saline control was given intravenously via a tail vein, at a volume of 1 mL/kg.
Protocol detail
Transient Focal Cerebral Ischemia Model
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Anesthesia
Animals
Candesartan
Food
Isoflurane
Middle cerebral artery occlusion
Reperfusion
Saline
Surgery
Suture
Tail
Vein
Corresponding Organization :
Other organizations : Augusta University, University of Georgia
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Variable analysis
independent variables
- Temporary (3 hour) middle cerebral artery occlusion (MCAO)
- Outcomes (not explicitly mentioned)
- Isoflurane anesthesia
- Single housing of animals before and after surgery
- Free access to food and water
- Positive control: Candesartan 1mg/kg administered intravenously at reperfusion
- Negative control: Saline control administered intravenously at reperfusion
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