ChIP-qPCR Analysis of NF-κB Pathway

ChIP was performed as described previously [66 (link),112 (link)]. Anti-NF-κB p65 (SC-372, Santa Cruz Biotechnology, Dallas, TX, USA), (3660S, Cell Signaling, Danvers, MA, USA), anti-NCoR (17-10260, Merk Millipore, Darmstadt, Germany), anti-HDAC3 (SC-17795, Santa Cruz Biotechnology, Dallas, TX, USA), and normal IgG (12-370, Merk Millipore, Darmstadt, Germany) were used. Real-time PCR was performed with a Stratagene Mx3000P qPCR machine (Agilent Technologies, CA, USA). Oligonucleotides are listed in Supplementary Table S2.

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Jeong H., Chong H.J., So J., Jo Y., Yune T.Y, & Ju B.G. (2022). Ghrelin Represses Thymic Stromal Lymphopoietin Gene Expression through Activation of Glucocorticoid Receptor and Protein Kinase C Delta in Inflamed Skin Keratinocytes. International Journal of Molecular Sciences, 23(7), 3977.

Publication 2022

Anti-NF-κB p65 (SC-372, normal IgG (12-370, Stratagene Mx3000P qPCR machine

Chip Ncor Nf κb p65 Oligonucleotides Real time pcr Sc 372

Corresponding Organization : Sogang University

Other organizations : Kyung Hee University

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Variable analysis

independent variables

Anti-NF-κB p65 (SC-372, Santa Cruz Biotechnology, Dallas, TX, USA)
Anti-NF-κB p65 (3660S, Cell Signaling, Danvers, MA, USA)
Anti-NCoR (17-10260, Merk Millipore, Darmstadt, Germany)
Anti-HDAC3 (SC-17795, Santa Cruz Biotechnology, Dallas, TX, USA)

dependent variables

Outcome of ChIP experiments

control variables

Normal IgG (12-370, Merk Millipore, Darmstadt, Germany)

positive controls

ChIP was performed as described previously [66 (link), 112 (link)]

negative controls

Normal IgG (12-370, Merk Millipore, Darmstadt, Germany)

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