Cytotoxicity Evaluation of Novel Compound

All materials and reagents were purchased from commercial sources and used as received. CPT, dimethyl sulfoxide (DMSO), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and trypan blue were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS), fetal bovine serum (FBS), penicillin/streptomycin, trypLE express enzyme, McCoy's 5A media and Ham's F-12K (Kaighn's) nutrient mix were purchased from Life Technologies (Carlsbad, CA, USA). Paclitaxel was sourced from 21 CEC PX Pharm Ltd., (East Sussex, UK); chloroform and hydrochloric acid, from APS Chemicals (Canning Vale, WA, Australia); and sodium hydroxide, from Ajax FineChem (Scoresby, VIC, Australia). P4C6 was synthesized in our lab to a purity of >95%, as confirmed by HPLC (Mo et al., 2015 (link)).