Compounds 2a2l were evaluated for anticholinesterase activities by the Ellman’s method [36 (link)] with slight modification as described in previous investigations [22 (link),37 (link),38 (link)]. The absorbances were determined spectrometrically at 412 nm on a Varioskan flash spectrophotometer (Thermo Scientific, Waltham, MA, USA). The IC50 and standard deviation values were determined graphically using Graph Pad Prism.
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