To determine apoptotic rates of MeHg-treated cells, MCF7 cells were treated with MeHg as described above. On day 5, the apoptotic rates were determined with an Annexin-V-Fluos staining kit according to the manufacturer’s instructions (Sigma-Aldrich, United States). In short, the cells were incubated in Annexin-V-FITC (Sigma-Aldrich, United States) for 30 min, washed 3x with HBBS, and analyzed by immunofluorescence [29 (link)].
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