Evaluating Mineralization Potential of Dental Cements

Mineralization or calcification ability of the tested CSMs on hPDLSCs were evaluated by Alizarin Red S staining after 21 days of culturing with undiluted (1:1) cement-conditioned medium, as follows: (A) control (DMEM), (B) Osteodiff, (C) Ceraputty, (D) ERRM, and (E) Biodentine. After the culture period, the samples were rinsed with fetal bovine serum and fixed with 70% ethanol for 1 h. Then, samples were stained with 2% Alizarin Red solution (Sigma Aldrich) for 30 min in controlled conditions (dark ambient and room temperature) and solubilized using 10% cetylpyridinium chloride monohydrate solution (Sigma-Aldrich). Finally, Synergy H1 multi-mode microplate reader (BioTek, Winooski, VT, USA) was used to measure the absorbance values of the samples at 570 nm. The methodology for Alizarin Red S staining assay was based on a previous similar study [21 (link)].