Vitamin D3 Bioconversion Assay

This was carried out by pooling the fractions with main vitamin D₃ bioconversion activities into one fraction (100 mL) which was then divided into four aliquots (each of about 25 mL). Protein contents of these aliquots were recovered, by ammonium sulfate precipitation, and treated for testing their vitamin D₃ bioconversion activities, as previously described, except that:
(і) One aliquot coded A, was subjected to no ammonium sulfate addition.
(іі) The three remaining aliquots, coded A2%, A5%, and A10%, were subjected to ammonium sulfate additions at 2, 5, and 10% w/v concentrations, respectively.
The results obtained were recorded and compared.