Bone Marrow Cell Culture Protocol
Corresponding Organization : Tianjin Medical University
Variable analysis
- Presence of the indicated growth factors: 100 ng/ml murine SCF, 50 ng/ml murine IL6, 100 ng/ml murine TPO, and 100 ng/ml murine FLT3L
- Outcome of BM cultures, which are not explicitly mentioned
- 2 × 10^5 cells of total nucleated BM cells
- Iscove's Modified Dulbecco's Medium (IMDM) medium with 10% serum
- Culture duration of 10 days
- No positive or negative controls were explicitly mentioned.
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