Human lung cancer cell line A549 (BFN60800665,
BLUEFBIO™, China), H1299 (BFN60804058,
BLUEFBIO™, China) and human bronchial epithelial
cell line Beas-2B (BFN608009328, BLUEFBIO™,
China) were cultured in Dulbecco’s modified Eagle’s
medium (DMEM, Cat No. C11995500BT, Gibco,
China), supplemented with 2 μM of L-glutamine (Cat
No. 25030149, Gibco, China), 25 μg/ml of gentamycin
(Cat No. 15710-049, Thermo Fisher Scientific, China),
and 10% heat-inactivated fetal bovine serum (FBS,
Cat No. 10099-141C, Gibco, China). Cell transfections
were performed by using PolyJetTM DNA In Vitro
Transfection Reagent(Cat No. SL100468, SignaGen
Laboratories, USA), according to the manufacturer’s
instruction and described in the previous studies (18 (link)).
For the transfection of pcDNA3.1/Flag-FHL1 into A549
and H1299 cell lines, 2 μg of plasmids were used, and
the stable transfectants were generated by G418 selection
(500 g/ml). For the transfection of shRhoGDIβ into A549
(Flag-FHL1), 2 μg of plasmids were used, and the stable
transfectants were generated by puromycin selection (2
μg/ml).
BLUEFBIO™, China), H1299 (BFN60804058,
BLUEFBIO™, China) and human bronchial epithelial
cell line Beas-2B (BFN608009328, BLUEFBIO™,
China) were cultured in Dulbecco’s modified Eagle’s
medium (DMEM, Cat No. C11995500BT, Gibco,
China), supplemented with 2 μM of L-glutamine (Cat
No. 25030149, Gibco, China), 25 μg/ml of gentamycin
(Cat No. 15710-049, Thermo Fisher Scientific, China),
and 10% heat-inactivated fetal bovine serum (FBS,
Cat No. 10099-141C, Gibco, China). Cell transfections
were performed by using PolyJetTM DNA In Vitro
Transfection Reagent(Cat No. SL100468, SignaGen
Laboratories, USA), according to the manufacturer’s
instruction and described in the previous studies (18 (link)).
For the transfection of pcDNA3.1/Flag-FHL1 into A549
and H1299 cell lines, 2 μg of plasmids were used, and
the stable transfectants were generated by G418 selection
(500 g/ml). For the transfection of shRhoGDIβ into A549
(Flag-FHL1), 2 μg of plasmids were used, and the stable
transfectants were generated by puromycin selection (2
μg/ml).
