Immunoblotting Antibodies and Reagents

Antibodies used for immunoblotting are as follows: Rat α-FLAG L5 (#637303; BioLegend), Rabbit α-HA, Rabbit α-GFP, Rabbit α-Sec62, Rabbit α-Sec63, Rabbit α-Sec61β, Rabbit α-BAG6, and Rabbit α-Sec61α (Chitwood et al., 2018 (link); Mariappan et al., 2010 (link); Snapp et al., 2004 (link)) are a gift from Dr. Ramanujan Hegde (MRC Laboratory of Molecular Biology, Cambridge, UK). Rabbit α-BiP (#11587-1-AP; Proteintech), Mouse α-HA (#901513; Biolegend), Mouse α-PDI (#MA3-018; Affinity Bioreagents), Goat α-Rat-HRP (#7077; Cell Signaling), Goat α-Mouse-HRP (#115-035-003; Jackson ImmunoResearch), Goat α-Rabbit-HRP (#111-035-003; Jackson ImmunoResearch), Goat anti-rat HRP (#7077S; Cell signaling), Goat α-RAT IgG-Cy2 (#112-225-167; Jackson ImmunoResearch), Goat α-Mouse IgG-Alexa657 (#A-21235; Invitrogen). Beads were purchased as follows: Strep-Tactin XT beads (#2-4010-010; IBA), Rat anti-FLAG L5 affinity gel (#651503; Biolegend), Protein A agarose (#CA-PRI-0100; Repligen), Mouse anti-HA magnetic beads (#88837; Pierce), Poly L-lysine (#OKK-3056; Peptides International). Rabbit Reticulocyte Lysate was purchased from Green Hectares (Ph:1-800-GHLYSAT). Detergents were purchased as follows: digitonin (EMD Millipore), Triton X-100 (Thermo Fisher Scientific), sodium deoxycholate (Sigma-Aldrich), SDS (Sigma-Aldrich), and Tween 20 (American Bioanalytical).

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Sun S., Li X, & Mariappan M. (2022). Signal sequences encode information for protein folding in the endoplasmic reticulum. The Journal of Cell Biology, 222(1), e202203070.

Publication 2022

Mouse α-HA Mouse α-PDI Goat α-Mouse-HRP Goat α-Rabbit-HRP Goat anti-rat HRP Goat α-RAT IgG-Cy2 Goat α-Mouse IgG-Alexa657 Protein A agarose Poly L-lysine digitonin Triton X-100 sodium deoxycholate SDS

Agarose Antibodies Detergents Digitonin Goat Lysine Mouse Peptides Poly Protein a Rabbit Reticulocyte Sodium deoxycholate Strep Triton x 100 Tween 20

Corresponding Organization : Yale University

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Variable analysis

independent variables

Antibodies used for immunoblotting
Beads
Detergents
Rabbit Reticulocyte Lysate

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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