The PAX3-FOXO1 fusion cDNA was subcloned into the lentiviral doxycycline-inducible pINDUCER10 vector (provided by Dr. J. Luo). A single-guide RNA (sgRNA) targeting the 5′ PAX3 region was designed (Table S1 ) and cloned into the lentiCRISPR v2 plasmid (provided by Dr. J. Luo) [13 (link)–15 (link)]. Oligonucleotides were purchased from Integrated DNA Technologies. Lentiviral particles were prepared in HEK293T cells with the HIV-based pPACK-H1 Packaging Plasmid Mix (System Biosciences).
Protocol detail
Doxycycline-Inducible PAX3-FOXO1 Fusion Knockdown
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Center for Cancer Research
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- PAX3-FOXO1 fusion cDNA subcloned into the lentiviral doxycycline-inducible pINDUCER10 vector
- Single-guide RNA (sgRNA) targeting the 5' PAX3 region cloned into the lentiCRISPR v2 plasmid
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!