TRPV4 Agonist and Antagonist Effects

TRPV4 agonist GSK1016790A (GSK) was purchased from Selleckchem (Houston, TX, Cat. #S6637). TRPV4 antagonist HC067047 (HC) was purchased from Sigma (Saint Louis, MO; Cat. # SML0143). Because TRPV4 can be activated by several stimuli, including heat and mechanical stress (Nilius et al., 2004 (link)), we avoided temperature changes and fast superfusion to study the separate effects of the chemicals. Thus, experiments were conducted at room temperature (22°C–24°C); agonist and antagonist were diluted in external solution and gently dripped onto the recording chamber for final concentrations of 10 nM GSK1016790A and 10 µM HC067047. For TRPV4 inhibition, cells were preincubated with HC067047 at room temperature for at least 30 min before the start of recordings.