Triterpenoid CDDO-EA Anti-inflammatory Protocol

The synthetic triterpenoid 2-cyano-3,12-dioxooleana-1,9(11 (link))-dien-28-oic acid (CDDO) along with chemically modified derivatives, CDDO-Me (methyl ester, also known as bardoxolone methyl, BARD) and CDDO-EA (ethyl amide) are non-cytotoxic, highly multifunctional and orally available drugs that have been studied as anti-inflammatory and anti-oxidation agents in vivo and in vitro (43 (link)–45 (link)). Because ethyl amide derivative of a synthetic triterpenoid, CDDO-EA, has been reported to have enhanced pharmacodynamic activity in mouse assays compared to CDDO-Me (46 (link)), CDDO-EA (400 mg/kg diet) (provided from Reata Pharmaceuticals, Irving Texas, and Dr. Michael Sporn, Dartmouth Medical School, NH, USA) was first dissolved in an oily vehicle and prepared into chow pellets (Lab Diet #5002) by Purina (Purina-Mills, Richmond, IN, USA) (47 (link)). Group of mice were continuously fed a CDDO-EA diet or Control diet (Lab Diet #5002) for 3 days prior to radiation exposure.

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Kim S.B., Bozeman R., Kaisani A., Kim W., Zhang L., Richardson J.A., Wright W.E, & Shay J.W. (2015). Radiation Promotes Colorectal Cancer Initiation and Progression by Inducing Senescence-Associated Inflammatory Responses. Oncogene, 35(26), 3365-3375.

Publication 2015

CDDO-EA Lab Diet #5002

Acid Amide Anti agents Anti inflammatory Assays Bardoxolone methyl Cddo Derivatives Diet Ester Exposure to radiation Mice Oily Pellets Pharmaceuticals Triterpenoid

Corresponding Organization :

Other organizations : The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

CDDO-EA (400 mg/kg diet)

dependent variables

Anti-inflammatory and anti-oxidation effects in vivo and in vitro

control variables

Control diet (Lab Diet #5002)

controls

Positive control: Not specified
Negative control: Control diet (Lab Diet #5002)

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