Extraction and Preparation of Medicinal Herb Extracts

Dried herbs of A. ternata (AT), P. cocos (PC), and Z. officinale (ZO) were purchased from Herbmaul (Chungcheongbuk-do, South Korea). To prepare the extract, dried herbs (100 g) were ground to a powder and extracted with 500 mL in distilled water at 100 °C for 15 min. In addition, the mixed sample was blended with equal weights of AT, PC, and ZO (33 g each). The hot water extracts were filtered twice through 8 μm-pore-size Whatman filter paper, concentrated by rotary evaporation (Buchi, Flawil, Switzerland), and freeze-dried to obtain lyophilized extracts of AT, PC, and ZO. These were then eluted with DPBS and filtered through a 0.22 μm syringe filter before cell treatment.

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Kim T.H., Yu G.R., Kim H., Kim J.E., Lim D.W, & Park W.H. (2023). Network Pharmacological Analysis of a New Herbal Combination Targeting Hyperlipidemia and Efficacy Validation In Vitro. Current Issues in Molecular Biology, 45(2), 1314-1332.

Publication 2023

Whatman filter paper rotary evaporation

Cell Cocos Freeze Powder Syringe

Corresponding Organization : Dongguk University

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Variable analysis

independent variables

Type of dried herbs used (A. ternata, P. cocos, Z. officinale)
Extraction method (hot water extraction at 100°C for 15 min)

dependent variables

Characteristics of the lyophilized extracts (e.g., yield, chemical composition)

control variables

Mass of dried herbs used (100 g)
Volume of distilled water used (500 mL)
Filtration method (filtered twice through 8 μm-pore-size Whatman filter paper)
Concentration method (concentrated by rotary evaporation and freeze-dried)
Preparation of mixed sample (blended with equal weights of AT, PC, and ZO)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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