Western Blot Analysis of Fibrotic Markers

Western blot analysis was used to detect changes in Annexin A1 (LOT# sc-53158; Santa Cruz Biotechnology, Santa Cruz, CA, USA), collagen type I (LOT#14695-1-AP, Proteintech™), collagen type III (LOT#13548-1-AP, Protein-tech™), α-SMA (LOT#14968, Cell Signaling Technology), p-p38 antibody (LOT#4511, Cell Signaling Technology), p38 antibody (LOT#8690, Cell Signaling Technology), p-ERK1/2 antibody (LOT#4370, Cell Signaling Technology), and ERK1/2 antibody (LOT#4695, Cell Signaling Technology) as previously described.26 (link) Images were subsequently analyzed using Quantity One to quantify the protein expression (BioRad, CA, USA).

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Lai T., Li Y., Mai Z., Wen X., Lv Y., Xie Z., Lv Q., Chen M., Wu D, & Wu B. (2018). Annexin A1 is elevated in patients with COPD and affects lung fibroblast function. International Journal of Chronic Obstructive Pulmonary Disease, 13, 473-486.

Publication 2018

α-SMA p-p38 antibody p38 antibody p-ERK1/2 antibody ERK1/2 antibody

Annexin a1 Antibody Collagen type i Collagen type iii Erk1 Lot 1 Protein Western blot analysis

Corresponding Organization :

Other organizations : Guangdong Medical College

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Variable analysis

independent variables

Annexin A1 (LOT# sc-53158; Santa Cruz Biotechnology, Santa Cruz, CA, USA)
Collagen type I (LOT#14695-1-AP, Proteintech™)
Collagen type III (LOT#13548-1-AP, Protein-tech™)
α-SMA (LOT#14968, Cell Signaling Technology)
P-p38 antibody (LOT#4511, Cell Signaling Technology)
P38 antibody (LOT#8690, Cell Signaling Technology)
P-ERK1/2 antibody (LOT#4370, Cell Signaling Technology)
ERK1/2 antibody (LOT#4695, Cell Signaling Technology)

dependent variables

Protein expression

control variables

Western blot analysis procedure as previously described in the referenced study

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

Annotations

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