Protocol detail

Synthetic Histone H3.1 Tail PTM Analysis

Find Similar Protocols

A set of five histone H3.1 tail peptides (ARTKQTARKSTGGKAPRKQLATKAARK-SAPATGGVKKPHRYRPGTVALRE) was probed incorporating acetylation (AcK18 and AcK27) and trimethylation (TriMetK4, TriMetK9 and TriMetK27) PTMs in biologically relevant positions (MW 5381 Da).^{44 (link)} These histone tails are proteotypic for the endoproteinase Glu-C derived peptides from histone H3. The synthetic histone H3.1 tails were purchased from GenScript (Piscataway, NJ). H3.1 tail solutions were analyzed at a concentration of 5 μM in 50:50 water/methanol (H₂O/MeOH) solvent conditions. A low concentration tuning mix standard (G1969–85000, Agilent Technologies, Santa Clara, CA) was used for external mobility and mass calibration.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Dit Fouque K.J., Kaplan D., Voinov V.G., Holck F.H., Jensen O.N, & Fernandez-Lima F. (2021). Proteoform Differentiation using Tandem Trapped Ion Mobility, Electron Capture Dissociation, and ToF Mass Spectrometry. Analytical chemistry, 93(27), 9575-9582.

Publication 2021

G1969–85000

Acetylation Endoproteinase glu c Histone Histone h3 Methanol Mobility Peptides Ptms Solvent Tail

Top 5 similar protocols

Variable analysis

independent variables

Histone H3.1 tail peptides with different post-translational modifications (PTMs): acetylation (AcK18 and AcK27) and trimethylation (TriMetK4, TriMetK9 and TriMetK27)

dependent variables

Characteristics of the histone H3.1 tail peptides (e.g., mass, mobility)

control variables

Solvent conditions (50:50 water/methanol)
Concentration of histone H3.1 tail peptides (5 μM)
Low concentration tuning mix standard for external mobility and mass calibration

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!