Cultivation of E. coli O157:H7 Sakai

The strain of E. coli O157:H7 Sakai (19 (link), 20 (link)) used in this study was provided by Carlton Gyles (Guelph, Canada). A stock culture was maintained at −80 °C and was cultivated by plating onto brain-heart infusion (BHI) agar (Thermo Scientific, Waltham, MA, CM225) and incubating at 37 °C for 24 h. An isolated colony was inoculated into 25 ml of BHI broth and incubated at 37 °C for 20 h. The resulting broth culture was kept at 4 °C and used as a “working” culture within a week.

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King T., Kocharunchitt C., Gobius K., Bowman J.P, & Ross T. (2016). Physiological Response of Escherichia coli O157:H7 Sakai to Dynamic Changes in Temperature and Water Activity as Experienced during Carcass Chilling. Molecular & Cellular Proteomics : MCP, 15(11), 3331-3347.

Publication 2016

BHI) agar

Agar Brain E coli o157 Heart Strain

Corresponding Organization : Commonwealth Scientific and Industrial Research Organisation

Other organizations : University of Tasmania

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Variable analysis

independent variables

Strain of E. coli O157:H7 Sakai (19, 20)

dependent variables

Not explicitly mentioned

control variables

Cultivation of E. coli O157:H7 Sakai strain on BHI agar at 37 °C for 24 h
Inoculation of an isolated colony into BHI broth and incubation at 37 °C for 20 h
Storage of the resulting broth culture at 4 °C and use within a week

controls

Positive control: None mentioned
Negative control: None mentioned

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