Biomarker Profiling of Inflammatory Response

Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured using a dry chemistry analyzer (FUJI DRI-CHEM 7000V, FUJIFILM, Tokyo, Japan). To indirectly quantify endotoxin levels, lipopolysaccharide (LPS)-binding protein (LBP) concentrations were measured by ELISA according to the manufacturer’s guidelines (Guduo, Shanghai, China) because of the well-documented inaccuracies inherent to direct endotoxin measurements (Tremellen et al., 2017 (link)). Serum cytokine levels were measured using a Bio-Plex Mouse Cytokine 23-Plex Panel kit (Bio-Rad, Hercules, CA, United States) according to the manufacturer’s instructions. The following mouse cytokine levels were analyzed for each sample: interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17α, eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), gamma interferon (IFN-γ), keratinocyte-derived chemokine (KDC), macrophage chemoattractant protein 1 (MCP)-1, macrophage inflammatory protein-1α (MIP)-1α, MIP-1β, regulated upon activation normal T-cell expressed and secreted (RANTES), and tumor necrosis factor (TNF)-α.

Free full text: Click here

Ye J., Lv L., Wu W., Li Y., Shi D., Fang D., Guo F., Jiang H., Yan R., Ye W, & Li L. (2018). Butyrate Protects Mice Against Methionine–Choline-Deficient Diet-Induced Non-alcoholic Steatohepatitis by Improving Gut Barrier Function, Attenuating Inflammation and Reducing Endotoxin Levels. Frontiers in Microbiology, 9, 1967.

Publication 2018

DRI-CHEM 7000V Bio-Plex Mouse Cytokine 23-Plex Panel kit

Alanine aminotransferase Aspartate aminotransferase Chemokine Cytokine Elisa Endotoxin Eotaxin Gm csf Granulocyte colony stimulating factor Ifn γ Interleukin Interleukin 10 Interleukin 12 p70 Interleukin 13 Interleukin 1α Interleukin 1β Interleukin 2 Interleukin 3 Interleukin 4 Interleukin 5 Interleukin 6 Interleukin 9 Keratinocyte Macrophage chemoattractant Macrophage inflammatory protein 1α Mouse Protein 1 Serum T cell Tnf α

Corresponding Organization :

Other organizations : Zhejiang University, South China Institute of Collaborative Innovation, Wenzhou Central Hospital

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Alanine aminotransferase (ALT) levels
Aspartate aminotransferase (AST) levels
Lipopolysaccharide (LPS)-binding protein (LBP) concentrations
Serum cytokine levels (interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-17α, eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), gamma interferon (IFN-γ), keratinocyte-derived chemokine (KDC), macrophage chemoattractant protein 1 (MCP)-1, macrophage inflammatory protein-1α (MIP)-1α, MIP-1β, regulated upon activation normal T-cell expressed and secreted (RANTES), and tumor necrosis factor (TNF)-α)

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!