3D Reconstruction of Neuroblast Chains

Observation with SBF-SEM and analyses of acquired data were performed as described previously with slight modifications (53 (link), 54 (link)). SBF-SEM of the neuroblast chains was performed using a Merlin scanning electron microscope (Carl Zeiss) equipped with a 3View in-chamber ultramicrotome system (Gatan, Pleasanton, CA, USA). Sequential images were processed using FIJI. Segmentation of the cell membrane was performed using Microscopy Image Browser (55 (link)). 3D reconstruction was performed using Amira software (Visualization Sciences Group). For details, see the Supplementary Materials.

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Kaneko N., Herranz-Pérez V., Otsuka T., Sano H., Ohno N., Omata T., Nguyen H.B., Thai T.Q., Nambu A., Kawaguchi Y., García-Verdugo J.M, & Sawamoto K. (2018). New neurons use Slit-Robo signaling to migrate through the glial meshwork and approach a lesion for functional regeneration. Science Advances, 4(12), eaav0618.

Publication 2018

Merlin scanning electron microscope 3View in-chamber ultramicrotome system

Cell membrane Merlin Microscopy Reconstruction Sbf sem Scanning electron microscope Ultramicrotome

Corresponding Organization : Jichi Medical University

Other organizations : Ho Chi Minh City Medicine and Pharmacy University

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Variable analysis

independent variables

Observation with SBF-SEM

dependent variables

Acquired data from SBF-SEM

control variables

Experimental procedures were performed as described previously with slight modifications

controls

No positive or negative controls were explicitly mentioned in the input.

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