Validation of Pancreatic Cancer Cell Lines

Pancreatic cancer cell lines Capan-2, T3M4, PATU8902 and CFPAC have been already described (36 (link)). Cell lines were validated by STR profiling by the Genetics Core at the University of Arizona. All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, St Louis, MO USA) supplemented with 10% fetal bovine serum (FBS), 100 I.U/ml penicillin, 100 μg/ml streptomycin, and incubated at 37°C in a humidified incubator with 5% CO₂. Thapsigargin was purchased from Cell Signaling Technology (Danvers, MA USA) and stocks were diluted in DMSO. Tetrahydrouridine (THU) was purchased from Cayman Chemical (Ann Arbor, MI USA), Leflunomide was from Enzo Life Sciences (Farmingdale, NY USA). Deoxycytidine and deoxyuridine were obtained from MP Biomedical (Irvine, CA USA) and Sigma-Aldrich, respectively.