Healthy BALB/c background mice were purchased from the Jackson Laboratory. TxA23 mice express a transgenic T cell receptor specific for a peptide from H+/K+ATPase alpha chain on a BALB/c background and have been previously described.28 (link)29 (link)31 (link)32 (link) All mice were maintained in our animal facility and cared for in accordance with institutional guidelines. Studies were performed on a mixed group of male and female mice with cohoused littermate controls. The use of high dose tamoxifen to induce parietal cell atrophy and SPEM development has been previously described.20 (link)21 (link)33 (link) Briefly, tamoxifen (5 mg/20 g body weight, T006000, Toronto Research Chemicals) was injected intraperitoneally into 6–12 week old BALB/c mice once daily for 3 days. tamoxifen was dissolved in a vehicle of 10% ethanol and 90% sunflower oil (S5007, Millipore Sigma).
Protocol detail
Tamoxifen-Induced Parietal Cell Atrophy
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Animal
Atrophy
Balb c mice
Body weight
Ethanol
Female
H k atpase
Male group
Mice
Parietal cell
Peptide
Spem
Sunflower oil
T cell receptor
Tamoxifen
Transgenic
Corresponding Organization : Saint Louis University
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Variable analysis
independent variables
- Dose of tamoxifen (5 mg/20 g body weight)
- Parietal cell atrophy
- SPEM development
- BALB/c background mice
- TxA23 mice expressing a transgenic T cell receptor specific for a peptide from H+/K+ATPase alpha chain on a BALB/c background
- Age of mice (6-12 weeks)
- Route of tamoxifen administration (intraperitoneal injection)
- Tamoxifen vehicle (10% ethanol and 90% sunflower oil)
- Positive control: Tamoxifen-treated BALB/c mice to induce parietal cell atrophy and SPEM development
- Negative control: Not explicitly mentioned
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