RIP140-expressing vectors (pEFcmyc-RIP140 [47 (link)], pEGFP-RIP140[48 (link)]) and control vectors, pEGFP (Clontech), are described elsewhere. HA-HIF1alpha-pcDNA3 (Addgene plasmid # 18949) and HA-HIF2alpha-pcDNA3 (Addgene plasmid # 18950) plasmids were gifts from Dr Kaelin [49 (link)]. The GLUT3-Luc reporter gene was a gifts from Dr. Yuan [50 (link)]. 2-Deoxy-d -glucose (2DG, D6134)), 6-aminonicotinamide (6AN; # A68203), 3-bromopyruvate (BrP; #16490), MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) (98%, CAS 298-93-1), deferoxamine mesylate salt (D9533), di(N-succinimidyl)glutarate (80424), crystal violet (C0775), anti-Mouse IgG-FITC antibody (F6257), monoclonal Anti-β-actin-peroxidase antibody (A3854) were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany). Puromycin (ant-pr-1) was purchased from Invivogen (France). The ATP Determination Kit (10700345) was from Fisher Scientific (France). Deoxy-d -glucose, 2-[1,2-3H (N)] (NET328A250UC) was from Perkin Elmer (France). GLUT3 shRNA (m) (sc-41219-V) were purchased from Santa Cruz Biotechnology (Dallas, USA). Rabbit polyclonal to RIP140 (ab42126) was from Abcam (Cambridge, UK). BrdU Hu-purified-clone B44 (#347580) was from Becton Dickinson (France). Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France).
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